Abstract
Conclusions
Propagation of human or simian strains of poliomyelitis virus in any kind of culture medium is extremely difficult whereas certain murine strains will grow well in tissue culture containing embryonic mouse brain. A number of enteric bacteria and protozoa as well as some highly differentiated non-intestinal protozoa were examined for their ability to permit propagation of MM murine poliomyelitis virus in culture. No evidence of viral propagation was found as determined by mouse inoculation. The data do not encourage the assumption that neu-rotropic poliomyelitis virus in stool or sewage may be maintained in symbiosis with free living microbial cells. However the limited extent of this work does not exclude the possibility that experiments with other marine or faecal organisms under different experimental conditions might be more successful.
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