Abstract
Summary
A modified procedure for the preparation of relatively large batches of acetone-desiccated horse brain powder has been developed. The thromboplastic activity was influenced by the concentration of brain powder used for the preparation of the saline extracts. The optimal mixture was a 6% suspension, whose supernatant quite often had optimal activity after dilution with distilled water. The optimal temperature for the preparation of saline extracts was 5° C; the optimal period of exposure to this temperature was 15 to 30 minutes. Exposure to temperatures between 50° C and 100° C for 15 to 30 minutes produced only a partial destruction of thromboplastic activity.
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