Abstract
In a previous communication 1 we have described a method for quantitatively separating leucin and isoleucin from valin, the leucin isomers being precipitated as normal lead salts. It has since been found that from the specific rotation of the mixture of leucin and isoleucin, as obtained analytically pure from the lead salts, the percentage of each can be accurately calculated. The rotation of d-leucin in 20 per cent. hydrochloric acid is + 36.8°, of l-leucin + 15.6°. Consequently the proportions are calculated by the formulæ:
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