Direct Isolation of Influenza Virus in Chick Embryos

Abstract

Primary isolation of influenza virus in chick embryos has become a useful technique in the past several years largely through the work of Burnet on the advantages of inoculation into the amniotic sac,¹ and through the use of hemagglutinins as a means of detecting the virus.^{2, 3} Recently, Rickard, Thigpen, and Crowley⁴ have shown that influenza virus may be isolated by inoculation of untreated throat washings into the allantoic sac.