Abstract
The serum of rabbits bearing the growths of infectious papillomatosis and of rabbits immunized with purified virus or wart extracts has been studied 1 by the electrophoretic procedures of Tiselius. Differences from the normal electrophoretic patterns were seen only in the serum of animals actually carrying growths. In the present work studies were made on saline extracts of the virus-induced growths of domestic and cottontail rabbits.
The growths had been present for 39 to 115 days and, except for retrogressing warts in one animal, were exuberant and fleshy. Growths were removed from rabbits killed by air injection and, freed of connective tissue, were pulped in a Waring Blender and extracted for 24 hr at 2-8°C in 20% suspension in 0.9% NaCl solution. The extracts were filtered with celite and dialyzed against buffer prepared as in the serum studies. 1
In Fig. 1 is shown the electrophoretic diagram of a filtered extract of domestic rabbit papillomas showing 3 distinct migrating peaks, the mobilities of which were 5.73, 3.07, and 1.22 cm 2 /sec. volt, respectively. These values corresponded fairly closely to the mean mobilities found for albumin, beta globulin and gamma globulin of normal rabbit serum, 2 namely 5.13, 2.98 and 0.92, respectively. The greatest difference was seen with the peak in the relative position of the gamma globulin of serum diagrams. It has been noted that when filtered extracts are heated to 56°C for 30 min some of the protein is coagulated, a phenomenon not observed when serum is so heated. The diagram obtained with extract of Fig. 1 after being heated in this way is shown in Fig. 2. The protein coagulated and removed was 43% of the total present in the unheated extract. In Fig. 2 are seen again 3 chief components, the mobilities of which were, 5.20, 2.78, and 1.04 cm 2 /sec. volt.
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