Abstract
Serum from domestic rabbits bearing the growths of infectious papillomatosis 1 and those injected with extracts of domestic or cottontail rabbit growths or with the purified papilloma virus protein may contain immune bodies demonstrable by complement fixation, neutralization or precipitation. 2 Recently, studies have been made in this laboratory to learn whether these immunological characters are paralleled by abnormal electrophoretic behavior of such serum.
Sera from individuals of 6 groups of rabbits were examined: (1) domestic rabbits injected intraperitoneally with 0.5 mg of purified papilloma virus protein, once a week for 6 weeks, and serum taken for study every 7 days (for a total of 51 days); (2) domestic rabbits injected intravenously with 0.5 mg of purified papilloma virus protein, twice a week for 6 weeks, and the serum examined after 21 and 50 days; (3) domestic rabbits injected intraperitoneally with 1.0 cc of a 7.5% suspension of domestic rabbit warts, twice weekly for 6 weeks; (4) domestic rabbits bearing experimentally-induced growths for 39 to 72 days; (5) domestic rabbits carrying transplanted growths 3 in abdominal organs for 115 days; (6) cottontail rabbits bearing experimentally-induced growths for 37 and 40 days. The serum was diluted with 2 volumes of buffer solution of pH 7.8 containing 8.7675 g NaCl, 2.6274 g Na2HPO4, and 0.2071 g NaH2PO4 · H2O per liter and dialyzed for 4 to 6 days at 2 to 9°C. For electrophoresis the moving boundary method of Tiselius was used and the boundary gradients were recorded as previously described. 4 The index of serological change was complement-fixing titer.
In the sera of animals receiving the virus intravenously or intra-peritoneally, the complement-fixing titer was 1 :16 to 1 :256.
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