Abstract
Summary
Small pieces of heart tissue from young chick embryos coalesce to form a spherical body when brought into actual contact with each other in a mixture of chicken serum and Ringer's solution. The pieces must touch each other. A preliminary treatment of these fragments with “trypsin” increases the rate and percentage of coalescence. The method is serviceable for titration of cyto-antibodies in a medium, as these inhibit the coalescence in low concentrations.
The method is described, and its use is demonstrated by examples.
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