Physiological Observations Upon Larval Eustrongylides. III. Culture Attempts in vitro Under Sterile Conditions

Abstract

Summary

1. The maximal survival in vitro of an individual Eustrongylides larva at 37.5°C was 346 days. The medium was Bacto Yeast extract 0.5% + NaCl 0.5% + Glucose 0.5%. 2. The maximal average survival of a series of worms was 157 days in a medium containing Bacto-broth 0.8% + NaCl 0.5% + Glucose 0.5%. 3. The molecular concentration of the media could be varied in rather wide limits without material change in the length of survival. 4. The presence of 0.5% glucose was decidedly beneficial. 5. The worms produced acids. 6. No moulting or other sign of development was observed.