Abstract
Summary
1. A comparison of various methods for the injection of fertilized and incubated avian eggs has been conducted. The results were similar in all instances wherein the injection was made upon the chorio-allantoic membrane, regardless of the method used to approach the tissue membrane. 2. It was found that large quantities of distilled water, physiological saline, Tyrode's solution and F.D.A. broth could be injected into the egg without affecting the embryo's vitality. Eggs were shown to be capable of sustaining injections of at least 2 cc with maintenance of vitality during at least 48 hours' incubation at 38.5°C following injection. 3. Minimum lethal doses for the avian-embryo were established for the bactericidal agents under test, employing 9- and 12-day-old chick-embryos. The older embryos displayed higher resistance to the toxic substances than did those of 9 days age. 4. Bactericidal efficiencies of the bac-tericides under test were conducted by the Food and Drug Administration technic, against Staphylococcus aureus. In addition, all dilutions of the bactericidal agents were subcultured into another test tube of F.D.A. broth to test for bacteriostasis. 5. A number known as the “Toxicity Index” was determined from a comparison of the bactericidal efficiency against Staphylococcus aureus, with the toxicity of the bactericide for chick-embryos.
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