Abstract
Conclusion
On the medium described above we have been able to cultivate Sp. gallinarum without difficulty. The cultures grow best at 37°C, but growth occurs also at 30°C and at 39°C; at 30°C growth is slower but the viability better than at 37°C, at 39°C the reverse is the case. The cultures reach optimum growth density in 4 to 5 days according to the size of the inoculum. If transferred to the ice box at this point they remain active for 10 days to 2 weeks. Transplants have been made as late as the 15th day of culture. One strain has been carried through 19 generations during a period of 150 days. The 18th subculture of this strain was still fully virulent for chickens and caused fatal infections.
Get full access to this article
View all access options for this article.