Abstract
Summary and Conclusions
Cl. welchii was grown in a medium free from peptone or other substances of large molecular weight. Toxins were consistently produced which were equal to or more potent than those produced in glucose peptone beef infusion broth. The pH of the culture media did not fall below 6.0. This may in part be responsible for the higher potency of toxin obtained in this medium. The basal medium alone did not support growth; when it was supplemented with pantothenic acid, pimelic acid, nicotinic acid, riboflavin, or liver-extract, there was a marked increase of growth and toxin-production.
The fact that the highest bacterial-nitrogen value was obtained in glucose-peptone beef-infusion broth, appears to indicate that the casein hydrolysate still lacks one or more chemical ingredients required for maximal growth.
The work is being continued in an effort to identify a chemically defined medium for maximal growth and consistent toxin production by Cl. welchii.
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