Abstract
Flexner's M.V. strain of poliomyelitis can produce paralysis in Eastern cotton rats. 1 Another experiment was started on 8/13/40 to see if our previous results could be duplicated. 1 The usual injections of cord brain suspension in these experiments were 0.06 cc intracerebrally (IC), 0.06 cc intranasally (IN) and 0.5 cc subcutaneously (SQ) of a 10% suspension in “enteric organism filtrate.” The starting injection in the first generation was made with monkey cord. Each subsequent generation got the rat cord and brain of the preceding generation. Ten animals in Generation (Gen.) I were injected. All died between the 16th and 30th day; none had paralysis. Three animals in Gen. II were injected on 8/29 with Gen. I rat which died on the 16th day. These animals died between 42 and 46 days. Three animals in Gen. Ill were injected on 10/16 with a combination of all 3 animals from Gen. II. This time an additional injection of 1.0 cc was made IP. Two animals died on the 3rd and 1 on the 5th day; all were weak; none had paralysis. Three animals in Gen. IV were injected on 10/21 with a combination of all 3 rats of Gen. III. Twenty per cent cord suspensions were used in this experiment. One animal died on the 2nd, 1 on the 7th and 1 on the 8th day; the latter 2 were weak. Gen. V: On 10/30, 2 animals were injected with a 10% saline suspension combination from Gen. IV—the second 2 rats above. One animal was weak on the 5th and 1 had definite paralysis on the 6th day. Gen. VI.: The 2 animals injected on 11/7 with Gen.
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