Abstract
Conclusions
The Western strain of equine encephalomyelitis has been successfully grown in whole minced chick embryo or chorioal-lantois in serum-ultrafiltrate and buffered salt solution (Simms') at room temperature and at 37° C.
The highest titers (10-5 to 10-7) have been obtained with the clear supernatant fluid of chick and serum-ultrafiltrate cultures maintained at room temperature. The titers of these cultures can be sustained with little decrease for as long as 10 and 15 days.
The Eastern strain of equine encephalomyelitis has been grown with a dilution activity of 10-7 in whole minced chick embryo in serum-ultrafiltrate at room temperature and 37° C.
A vaccine, containing very small amounts of protein, prepared from the clear supernatant fluid of serum-ultrafiltrate cultures protected guinea pigs against 1,000 to 10,000 m.l.d. doses of virus (Western strain).
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