Abstract
Comment and Summary
By paying special attention to a few fundamental characteristics, significant findings were noted which may have important practical implications. The concentration of the organisms in the cells of the yolk sac which can easily be separated by centrifugation would suggest a simple method for the removal of much undesirable inert materials contained in the yolk sac during the preparation of vaccine. The presence of these substances has been the main objection for employing this vaccine in human prophylaxis. Successful initiation of cultures from the blood of infected animals naturally leads to its possible application for diagnosis in patients, when test animals usually employed such as guinea pigs and white rats were not available. Preliminary studies in this connection have offered some indication as to its practical use. The fact that death is regularly produced in white mice by the yolk sac cultivated rickettsiae gives us an additional and convenient animal which may be employed for typhus studies. By its use, immunological and chemotherapeutic studies can be facilitated and extended.
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