Abstract
Conclusions
The virus of St. Louis encephalitis may be grown in a medium containing embryonic mouse or guinea pig brain in ox serum ultrafiltrate. Cultures of organs from adult mice fail to support growth of the virus. Incubation at room temperature produces higher titers (10-5) than incubation at 37°C (10-3). At both temperatures the attained titer remains almost unchanged for 10 days but shows a decrease after 15 days' incubation. The virus is present in the same concentration in the supernatant fluid as in the emulsified whole culture. Infected chorioallantoic membranes maintained in serum ultrafiltrate at room temperature support growth of this virus up to titers varying from 10-4 to 10-5.
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