Abstract
Summary
The concentration of capsular polysaccharide appearing in blood-broth culture filtrates following the growth of a highly virulent, standardized strain of Pneumococcus III, was determined photronreflectometrically and correlated with the growth rate of the organism. An initial inoculum of 165 organisms per ml yielded a very small quantity of polysaccharide (0.5 mg %). An inoculum of 9,000 organisms per ml yielded 18 mg % of capsular polysaccharide in 48 hours. The greatest increases in concentration of polysaccharide occurred in two stages after the completion of the logarithmic phase of growth.
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