Abstract
Summary
(1) Peptone-Dextrose broth (PD), made with currently available samples of Neo-, Proteose and Pfanstiehl peptones, has failed to support growth of beta hemolytic streptococcus strain C 203 at the elevated incubation temperatures which are required for demonstration of bactericidal activity with low concentrations of sulfanilamide. (2) A modified PD broth containing 2.0% Tryptose and 0.1% Pfanstiehl peptones together with 0.2% dextrose and 0.5% sodium chloride buffered at pH 7.2 has been found to be satisfactory for use in studies of bactericidal activity. (3) Results obtained with this modified broth confirmed the conclusions previously drawn in regard to the critical relationship between temperature and the bactericidal activity of sulfanilamide.
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