Abstract
Summary
By treating the agglutinate of Type I Pneumococcus with dilute HC1 (pH 2), all the antibody is liberated, and after removing the cells and neutralizing, as much as 87% may be recovered. The recovered antibody is at least 95% pure as shown by specific precipitation followed by agglutination. The vaccine can be used repeatedly for the isolation of the antibody. The method thus combines simplicity of procedure with good yield and high purity of product.
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