Abstract
Pure albumin and pseudoglobulin were prepared from dog's blood according to the method of Hektoen and Welker. 1 Potent precipitins were prepared for the above antigens according to the method of Hektoen and Welker 2 and were used to determine the presence or absence of blood-proteins in the urine of dogs in subsequent experiments.
Four normal dogs with normal urines were exsanguinated; the blood was saved to obtain blood proteins, and the liver, brain, stomach, spleen, lung and kidneys were perfused with saline, and then thoroughly ground. A portion of kidney and all of the other organ-materials were used to prepare autolysates, and the remainder of the kidney-material was washed several times with more saline, fixed on aluminium cream and injected into rabbits for preparation of organ-antiserum according to the method of Spinka and Weichselbaum, 3 whereby blood-protein antibodies are eliminated without the necessity of absorption.
The kidney-antisera cross-reacted with liver- and brain-autolysates though to a definitely lower titer than with the specific antigen.
Healthy dogs with normal blood pressure and urine were injected intravenously with normal rabbit serum, a dog-kidney antiserum which had become attenuated, and a potent kidney-antiserum in doses of 1 cc per pound of body weight. Urines were examined for albumin, red blood cells, and casts, and by means of precipitin-tests for kidney-protein and dog-blood protein.
The injection of normal rabbit serum and attenuated kidney-antiserum produce no demonstrable changes. The injection of potent kidney-antiserum produced within one hour blood-pressure changes, appearance of kidney-proteins, red blood cells, and granular casts in the urine, and the microscopic pathological changes of extreme diffuse vascular dilatation, increase of Bowman's space, branching of glomeruli, tubular degeneration, and leukocytic infiltration of the entire kidney.
Get full access to this article
View all access options for this article.