Abstract
Thiry 1 described his classical method for the study of intestinal functions. Vella 2 improved this preparation by transforming a blind Thiry pouch into a tube with openings at both ends available for study. From that time many modifications of this basic principle have been evolved. In each, however, the segment of gut lies within the peritoneal cavity so it is not possible for the experimenter to observe directly its movements. As such observations are highly desirable, a preparation has been made which differs from the classical in that the isolated segment of gut is taken out of the peritoneal cavity and placed directly under the skin. This allows not only more accurate control of balloon and bolus preparations, but also permits direct visual observations of the behavior of the gut, since movements are most easily seen through the thin overlying skin.
The operation is performed in the following manner. Through a midrectus incision a loop of intestine is selected whose mesenteric vessels are of sufficient length to allow the intestine to be brought freely to the exterior. A segment of 12 to 20 cm is isolated from this loop. The continuity of the remaining gut is restored by “end-to-end” anastomosis and the intestine returned to the peritoneal cavity. The rectus incision is now carefully closed by suturing the peritoneum and fascia around the mesenteric vessels of the remaining isolated segment which lies on the surface of the rectus. By blunt dissection sufficient skin is separated from the underlying fascia until the isolated gut can be housed in the space thus provided. This skin flap is then pulled over the gut and incisions made in it through which each end of the segment is drawn and sutured.
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