Abstract
Summary
A simple method is described whereby estrogenic substances can be extracted from human urine for bioassay. This method consists of adding 50 cc concentrated HCl (35-37%) per liter of urine; boiling for one hour and then cooling; vigorously shaking on a mechanical shaker for one hour with an equal volume of ethyl ether (U.S.P.); breaking up emulsion with 30-50 cc of sodium taurocholate (10% sol.) and discarding aqueous fraction; adding 6 cc sesame oil to the etherial extract and then evaporating ether on a hot water bath. This method is comparatively accurate and can be accomplished in about 3 1/2 hours, thus making it an excellent routine method in an endocrine laboratory to expedite clinical and experimental work.
Get full access to this article
View all access options for this article.