Abstract
Uterine bleeding in the rhesus monkey occurs after withdrawal of an adequate estrogenic stimulus. In routine treatments of 100 to 400 I.U. per day for 10 days, bleeding occurs in from 5 to 16 days after the last injection. In our experience, the most frequent day for bleeding of estrone withdrawal is day 7, or 17 days after the first injection.
The present series of experiments was performed with adult female macacus monkeys all of which were previously castrated. A single massive injection of an estrogen was given and the day on which uterine bleeding occurred was noted. Vaginal lavages were made daily in each animal from the time of the maximal sex skin response.
Treatment with estradiol benzoate oleosum (Progynon B Schering). A single injection of 150,000 I.U. (15 mg) of estradiol benzoate in oil was made. The site of injection in 2 monkeys was into the uterine cervix. By means of a vaginal speculum and adequate lighting 1 cc of the preparation was injected into the dense, fibro-muscular tissue of the vaginal portion of the cervix with a number 25 hypodermic needle. The site was cleansed before and after injection with alcohol. Due to the great density of the tissue it was necessary to make 5 or 6 separate injections into the cervix around the external os, the total dose of 1 cc thus being deposited in several pockets forced into the tissue by the pressure applied. If considerable resistance to injection does not occur, the needle is withdrawn, as a gland or the cervical canal has ken entered. The purpose was to place the oily solution only in the fibromuscular coat of the cervix.
The greatest estrogenic response of the sex skin, color and edema, occurred between days 9 and 15 after the injection, after which it underwent a progressive involution.
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