Abstract
Summary
Acetone and ether drying of prehypophyseal tissue from pregnant cattle was observed to result in a 60% loss of mammogenic hormone. Similarly mammogen was not precipitated to any extent from 86% alcohol at pH 5.7. The residues obtained from the vacuum distillation of 86% alcohol and from hot alcohol-ether extraction were potent in mammogen.
These observations indicate that mammogen has distinctly different chemical properties from the lactogenic and carbohydrate-metabolism hormones which are isoelectrically precipitated in alcohol. Mammogen appears to be extracted in lipid solvents and remains in solution at high concentrations of alcohol. I t can be recovered as an oily residue upon the vacuum distillation of the alcohol or ether-alcohol extracting solutions.
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