Abstract
Conclusion
For dependable ascorbic acid values, blood should be centrifuged, the plasma deproteinized, and the plasma-HPO3 filtrate titrated in immediate sequence after the blood is drawn. Whole blood which stands in a closed small phial, with a minimum air space, may be depended upon to give results of clinical value for 1/2 hour. The higher values obtained with bloods to which KCN has been added represent an enhancement due to the action of KCN upon the 2,6-dichlorophenolindophenol, and in no wise a more accurate determination of their ascorbic acid content. This is particularly true with blood of low ascorbic acid value. KCN does not prevent the loss of ascorbic acid from blood.
It is a pleasure to acknowledge our indebtedness to Mrs. Jessie Maaske for technical assistance, and to Mr. Herman Chinn for checking several titrations.
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