Abstract
With the benzidine color test recently described 1 the following glucuronates∗ have been tested; borneol glucuronic acid, menthol glucuronic acid, and glucuronic acid monobenzoate. When 20 mg. samples were employed the test was negative, whereas Bial's test was positive with 2 mg. except in the case of borneol glucuronic acid which showed a positive test with Bial's reagent only when large amounts were employed. Glucuronic acid and glucuronic acid lactone gave a positive test with more than 0.2 mg. In this respect the benzidine test resembles Bial's test.
The new test is based on the formation of furfural. This aldehyd gives a red color with the benzidine solution without boiling.
In view of the fact that glucuronic acid does not occur free in urine, but in combination with phenols, etc., the benzidine test may be used to differentiate between pentoses and glucuronates.
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