Abstract
In 1934 and 1935 with the help of Decherd, the authors studied the mobilization of creatine in experimental myocardial damage 1 and the creatine and glycogen content of normal and infarcted heart muscle of the dog 2 and noted very definite and at times striking changes. The present series of experimental ligations of branches of the left coronary artery of the dog were carried out for the purpose of studying the effect of obstruction of the blood flow upon the phosphates, calcium, potassium as well as creatine levels of the dog's heart muscle.
Twenty out of 24 experiments were satisfactory. The dogs were completely anesthetized with amytal. A tracheal cannula was sewed into place and the chest opened under air pressure. The pericardium was brought up, split open and anchored to the chest wall. The anterior descending branch of the left coronary artery was tied off just below its first visible branch. The dogs were sacrificed after varying intervals from one to 22 hours. Sections were taken from the left ventricular muscle outside and in the infarcted area. Section A of apparently normal left ventricular muscle and Sections B from the infarcted areas were minced and ground up with chemically clean sea sand. Both were extracted with 5% trichloracetic acid and analyzed for total phosphorus, for acid soluble phosphate P and inorganic phosphate P by a modification of Deniges' 3 method.
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