Some Properties of the Antigonadotropic Factor

Abstract

Summary

In the above experiments we pointed out the following properties of the antigonadotropic factor derived from blood: It is not destroyed by one hour's heating to 70°C. It is, however, destroyed at 80°C, and by being boiled up once in diluted solutions. Heated for one hour at 100°C. in an acetone dry powder preparation it will not be destroyed. It is destroyed by pepsin, trypsin and n/10 NaOH. It is, however not affected by n/50 NaOH, n/50 NH₄OH, n/10 HCl, 1% H₂O₂-solution, ultraviolet irradiation. It is soluble in 40% acetone, insoluble in 50% acetone. It does not dialyze through cellophane and cuprophane membranes. We cannot exert any influence upon the mechanism of the prolan-antiprolan effect by “masking” with 5% glycocoll solutions. We pointed out the difference between the gonadotropic and the antigonadotropic factor, as well as between the antigonadotropic and antithyrotropic factor.