Abstract
The permeability of the stroma of malignant tumors of mice, both transplanted and spontaneous, has been investigated by the intravenous injection of a variety of foreign sera and dyes. The relative amount of the sera localized in the tumor was determined by the use of immunological methods and the dyes could be easily detected by direct examination under water.
After the intravenous injection of 0.25 cc. sera from the horse, rabbit, or chicken, precipitation tests on the extracts of the tumor, various organs, and the blood showed that, in the early period after injection (24 to 48 hours), a larger amount of the foreign sera was in the blood and tumor than in any of the organs.
After 3 to 5 days the sera from chicken and rabbit had disappeared from the blood and were found only or mostly in the tumor. Horse serum persisted in the blood for at least 10 days, but the tumor tisstie always contained more of it than the blood.
The dyes which were tested are T. 1824, Pontamine Sky blue, Brilliant Vital red, Vital New red, Dianil blue, and others, diluted at 1:1,000. These were injected intravenously at the amount of 0.25 to 0.50 cc. and were all found to localize selectively in cancer tissue with comparatively little localization in organs such as the liver and lymph nodes, where they might be expected to accumulate. The localization in the tumor takes place rapidly, the dye being clearly detected in the growth within one half hour after the intravenous injection. The maximum accumulation is accomplished in 12 to 20 hours. A broad zone of skin around the tumor is also deeply stained. The first 2 of the above series seem to give the most clear-cut results. T. 1824, a dye allied to Trypan blue, was selected for more extensive investigation.
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