Abstract
Summary
The transmitting agent of leukemia of mice, presum-ably malignant leukocytes, is inactivated by rapid freezing to −30°C., but remains viable even at −70°C. when frozen slowly. Sarcoma tissue of mice can also be frozen to at least −70°C. without being inactivated, and can be preserved at this temperature with little or no subsequent deterioration during at least 56 days.
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