Abstract
Conclusions
1. Yellow fever virus, unmodified strains as well as those adapted to tissue cultures in vitro, can be successfully cultivated in developing chick embryo. 2. Direct transfers can be maintained by the use of the chorio-allantoic membrane, embryonic fluid, or a suspension of minced tissues of the embryo. 3. A method of direct inoculation of the embryo in the egg with a tuberculin syringe and fine needle has been found satisfactory for routine use. 4. The virus strains studied showed no evidence of modification in the relatively few passages so far made.
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