Abstract
Conclusion
A niethod has been described for extracting mammotropic hormone from sheep pituitaries involving its solubility in HC1-acetone and NH3-acetone, and its precipitation with isoelectric protein at pH 5.5. A fraction made by the same procedure hut precipitated at pH 6.5 has heen shown to contain relatively little mammotropin, and is identified in the following paper as the adrenocorticotropic hormone. The “local” intradermal squab test has several advantages over the “systemic” tests of Riddle, et nl., when only mammotropic hormone is to be tested for, but the latter test has the very great advantage of allowing one to assay for the gonadotropic, thyrotropic, adrenotropic and mammotropic hormones on the same bird.
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