Abstract
The important discovery of Masugi 1 that in rabbits and rats glomerular nephritis can be produced by intravenous injections of anti-kidney serum has been confirmed by Fahr 2 and his pupils Hemprich 3 and Weiss, 4 in rabbits; by Arnott, Kellar and Matthew, 5 in rabbits; and by Farr and Smadel, 6 , 7 in rats. It has been shown that the animals develop albuminuria (up to 30%, Esbach), hematuria, cylindruria, lipuria, moderate oliguria or occasionally anuria (in acute stages), moderate edema, rise in blood urea (up to 224 mg. %), rise in blood pressure, cardiac hypertrophy, and anemia, and that at autopsy the kidneys exhibit extensive glomerular lesions which closely resemble those of human glomerular nephritis.
Masugi injected 10 cc. of a 10 to 30% suspension of blood-free kidney juice of rabbits in saline solution into the peritoneal cavity of ducks, and that 18 to 30 times at intervals of about 5 days. Five to 8 days after the last injection serum was prepared, and after inactivation by heating to 56°C. for 30 minutes, was injected intravenously once or repeatedly into rabbits in doses of 5 to 15 cc. Most rabbits received more than 15 cc. of serum. Hemprich suspended the juice of 2 kidneys in 100 cc. of saline solution. Of this suspension, 10 cc. were injected into ducks 9 to 22 times at 4 to 8 days'intervals. The serum of these animals was injected into rabbits in doses of 3.5 to 26 cc. Most rabbits received more than 10 cc. of serum. Weiss improved the method by thoroughly squeezing the remnants of the ground up kidneys while preparing the juice. He produced glomerular nephritis already with doses of 3 to 7 cc. of ducks'serum, in most cases with 4 to 5 cc. Arnott, Kellar and Matthews, using the original method of Masugi, injected the ducks 25 to 40 times.
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