Abstract
We have previously reported 1 that injections of large amounts of oestrin into mature female rats produced corpora lutea in the ovaries of these animals equal in size to those of pregnant rats. Similar results have been reported by Hohlweg 2 and Selye, Collip and Thomson. 3 Other experiments 3 , 4 , 5 have demonstrated that injection of oestrin simultaneously with A.P.L. (anterior pituitary-like substance) enhances the luteinizing capacity of A.P.L. and its capacity to increase the ovarian weights of immature rats. Hisaw and associates 6 and Lane 7 have demonstrated that injection of oestrin stimulated the production on the luteinizing hormone in the anterior hypophysis. The data presented below support the view that combined injection of oestrin and A.P.L. exerts a much greater effect on the ovaries of immature female rats than do injections of the A.P.L. factor alone.
In the first series of experiments a group of immature female rats received a single injection of 25 units of A.P.L.† They were killed 10 days later after no further treatment. A second group, littermate sisters to those above, received a single injection of A.P.L. and beginning on the fourth day following the administration of A.P.L. daily injections of 200 units of oestrin,† until the tenth day following the injection of A.P.L., at which time the rats were sacrificed. As controls for these experimental groups a group of untreated littermate sisters were used.
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