Abstract
A “prolan-like” substance in the urines and blood sera of different mammals which has little or no action by itself, but which was augmented or “synergized” by the gonadotropic action of an hypophyseal extract has been reported by Evans, Simpson and Austin. 1 Cole and Hart 2 and Saunders and Cole 3 attributed an analogous activity in the blood sera of pregnant and non-pregnant mares to a “luteinizing” substance resembling that found in the pituitary.
The present paper reports the ability of extracts of liver, thyroid, milk, egg white, and lemon juice, to augment the action of pituitary extracts, and deals with the augmentive interaction of human male urine with various gonad stimulating preparations. Female rats 21 to 22 days old were used as test animals and augmentation was determined by the increased weight of the test rats'ovaries over that of the controls receiving gonadotropic extract alone (Table I).
The male urine preparation was a tannic acid precipitate subsequently extracted with aqueous pyridine, reprecipitated with acetone, and reextracted with water in a procedure like that described for pregnancy urine by Hellbaum, Fevold and Hisaw. 4
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