Abstract
Low amounts of menopause or castration prolan which are difficult to recognize by the usual tests can easily be recognized by combining this substance with definite amounts of pregnancy prolan. The level of pregnancy prolan chosen is a low one but is always somewhat in excess of the minimal effective level when administered by itself. By the employment of this synergic reaction, rats prove to be just as sensitive test objects as are mice for the recognition of menopause or castration prolan.
A single example may be given. The administration of a gram of the alcohol precipitate from the urine of a castrated woman injected into 3 immature female rats, gave ovarian weights averaging 26 mg. and small or medium follicles only. When combined with levels of pregnancy prolan which alone gave ovarian weights averaging 30 mg., the combination yielded ovarian weights averaging 150 mg. In this case the hormone could not have been recognized in rats without the synergic reaction. It is remarkable that the “synergic” ovaries consist predominantly of follicles.
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