Abstract
It has been reported from several sources 1 , 2 , 3 that glycosuria was obtained after the injection of anterior pituitary extract. Houssay 4 reports that daily injections of the extract into dogs cause hyperglycemia, glycosuria and ketonuria. Evans, et al., 5 observed glycosuria in 2 dogs treated with growth hormone. Barnes, Regan, Nelson 6 found that Amniotin injections reduced glycosuria in depancreatized dogs. Houssay and Biasotti 7 injected rats with the Evans and Simpson extract for growth. Five to 7 injections were given, intraperitoneally, once a day in doses of 10 cc. per rat. Glycosuria and glycemia occurred 2 to 3 days after the cessation of the injections.
In view of these researches it was thought worth while to try the effect of the various pituitary hormones upon the blood sugar level of the rat. Growth hormone (Squibb); pregnancy urine extract, Antuitrin S (Parke-Davis); theelin (Parke-Davis) and Antuitrin G (Parke-Davis Growth Hormone) were used. All the determinations were made by the Somogyi Micro Method 8 for blood sugar. The following summaries give the different experiments with their results.
Antuitrin S. Ten rats were injected daily, subcutaneously, with 1 cc. of the extract for 8 days. Blood sugar was determined 1 day and 4 days after the last injection. No change in the blood sugar level was found. With 4 other rats 2 were injected subcutaneously and 2 intraperitoneally with 1/4 cc. twice a day for 4 days. On the 5th day the blood sugars were normal.
Antuitrin G. Six rats were injected subcutaneously with 1/4 cc. per day. Blood sugars determined on the 3rd and 8th days were normal. The dose was then stepped up to 1/2 cc. Two days later the blood sugars were normal. Injections were continued but by the intraperitoneal route for 3 more days after which time the blood sugar level was still normal.
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