Abstract
Summary
Experiments are presented showing that it is possible by adsorption with kaolin and subsequent successive elutions with ammonia to obtain a potent suspension of vaccinia virus giving negative Esbach and ninhydrin tests and containing 1.0 to 2.7 mg. non-ammonia N per 100 cc. of fluid. The severity of the reaction produced by a given dilution of the original testicular suspension is always greater than that produced by an equal volume of the same dilution of the eluted pure virus. In most instances the eluates were active in as high a dilution as the original suspension. The vaccinating efficiency of the pure virus has not yet been tested, but it is anticipated that it may prove useful for intradermal vaccination, particularly because of the milder character of the reaction produced by the purified virus.
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