Abstract
In the course of a study of serum phosphatase activity in the rabbit, dog and rat, it was found that bleeding, diet, distemper, and starvation exert a marked influence upon the valueis obtained. Bodansky and Jaffe 1 have pointed out that a relationship exists between the type of diet, the nutritional state of the animal, and the plasma phosphatase activity. For convenience of presentation, data is given under the 4 respective headings. Serum phosphatase activity was determined according to the method of Bodansky. 2
1. The influence of bleeding upon serum phosphatase activity was determined in adult rabbits. The serum obtained from 25 cc. of blood, drawn from the heart of rabbits, gave average values in a group of 14 animals for calcium of 13.96 mg./100 cc, for inorganic phosphorus 5.04 mg./100 cc, and for phosphatase activity 4.14 mg./100 cc. A second 25 cc sample of blood drawn 8 to 15 days later showed the influence of the previous bleeding upon serum constituents of otherwise normal rabbits maintained on the same diet. The values for serum calcium averaged 14.13 mg./100 cc, for inorganic phosphorus 3.74 mg./100 cc, and for phosphatase activity 2.25 mg./100 cc. These data show that bleeding caused a marked decrease in serum phosphatase.
2. The influence of a high protein or carbohydrate diet on the serum phosphatase activity was studied. The diet of a number of dogs was alternated from bread and meal mash to one of beef hearts. The influence of these 2 diets on the serum phosphatase and inorganic phosphorus is shown in Table I.
Get full access to this article
View all access options for this article.