Abstract
In a former communication 1 reactions with immune sera produced by injections of heteroalbumose preparations were briefly described. In order to give some additional information the following experiments, which were carried out with 3 albumose fractions obtained from the products of peptic digestion of coagulated sheep serum, may be cited.
Heteroalbumose was prepared in the manner previously described. 2 The preparation was precipitable from 1 % saline solution by about 33% alcohol or by one-half volume of saturated ammonium sulfate solution.
After removal of heteroalbumose, the albumoses which were precipitated between 1/2 and 2/3 saturation with ammonium sulfate were further fractionated at icebox temperature by precipitation with 65% alcohol at pH 5.1, fraction 1 being insoluble, fraction 3 remaining in solution. Fraction D 1 was precipitable by alcohol in a concentration above 50%, or by addition of 1 to 1.25 volumes of saturated ammonium sulfate solution. Fraction D 3 was precipitable by alcohol in a concentration above 80%, or by addition of 1.25 to 1.5 volumes of saturated ammonium sulfate solution.
Precipitin tests with a particularly strong antiserum obtained by injections of crude heteroalbumose mixed with charcoal 3 are given in Table I.
As already stated, by means of inhibition tests reactions could be demonstrated with various albumose fractions some of which were precipitable only to a very slight degree by the immune sera. Such tests are presented in Table II. Cross tests with albumoses from pig serum proved that the precipitation reactions as well as the inhibition effect are distinctly specific.
It may be mentioned that the inhibition phenomenon was observed with albumoses which were readily dialyzable through collodion membranes.
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