Abstract
In this laboratory we have been engaged in the preparation of potent extracts of the anterior pituitary for isolation of the lactation principle. While studying the effects of these extracts on the normal dog, the development of a severe lipemia was noted to occur some 24 to 48 hours after the first injection. Further investigation revealed that these extracts contained the diabetogenic principle of the anterior pituitary.
The writer communicated his findings to Dr. A. J. Carlson March 26, 1933, for criticism. Further, Dr. B. O. Barnes reported to the Physiological Society, Cincinnati meeting, April 11, 1933, that an acid extract of the anterior lobe produced glycosuria in 3 normal dogs. At that time, neither Dr. Barnes nor myself were aware of Houssay's report of the same nature. Thus, it seems that this observation was made independently in 3 laboratories, although admittedly earlier by Houssay. 1
The summary of the author's evidence that a diabetic syndrome of varying intensity can be established in normal dogs by the subcutaneous administration of alkaline extracts of the anterior lobe follows. To date 12 dogs have been studied.
Lipemia : Within 24 to 48 hours after the first injection, a marked lipemia developed in 6 of the 12 dogs. In general, this lipemia did not persist beyond the fifth day. It may be that this lipemia is caused by a sudden unloading of the fat stores of the body into the blood stream.
Polydipsia: Within 24 to 36 hours, polydipsia develops, all dogs drinking about 1900 cc. per day. It was distinctly observed that the polydipsia preceded the polyuria.
Polyuria: Polyuria is not marked until the fifth day, the urine output averaging then about 1500 to 2300 cc.
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