Abstract
In our recent paper 1 on the cultivation of the typhus fever rickettsiae. it was stated that “of several tissues tested, only tunica and peritoneum gave satisfactory results in the medium described.” Since then, we have succeeded in obtaining good growth with guinea pig testicle and kidney tissues in the serum Tyrode medium. Multiplication also occurred when various other tissues (guinea pig brain, heart, adrenal, lung, spleen, embryo) were used.
In these experiments, phenol red was added to the Tyrode solution in order to follow changes in reaction, as produced by the tissues during the incubation of the cultures. By reducing the amount of tissue in the medium, it was possible to retard the change in reaction, a circumstance which in our opinion may be significant in obtaining positive results.
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