Abstract
In work with parabiotic rats it becomes necessary to know something of the extent of blood transfer from one animal to its parabiont. Several authors (Martins, 1 Kallas, 2 Kawashima, 3 and others) have proven blood exchange through physiological or chemical tests, none of which, however, seem quantitative. The author 4 found much variation in different pairs and in the same pair at different times. The present work is an attempt to discover possible factors involved in such variations.
The dye, 1% Brilliant Vital Red (Evans), was used in Locke's solution. The adrenalin (hydrochloride compound of 1:1000 strength, preserved in 1% chloretone) was furnished gratis by the Parke, Davis and Company Research Laboratories. Chloretone was removed prior to use. The volume of adrenalin injected was 0.025 cc. and of dye 0.2 cc.
Control tests were run on each pair of rats by the method previously described (loc. cit.). The percentages expressed in this paper are based on the relative amounts of dye in each of the 2 animals at the end of one hour.
Experiment I. Dye and adrenalin were injected simultaneously in the tail vein of the left twin (all injections made directly into the tail vein). Immediately afterward adrenalin alone was injected in the right animal.
Experiment II. The right animal of each pair was not injected at all. Adrenalin mixed with dye was injected into the left member.
Experiment III. Adrenalin was injected in the right animal, the left twin receiving dye solution only.
Experiment IV. Dye was injected into the left twin at an interval of one minute after the same animal had received adrenalin. The right animal was not injected at all. The results of these experiments are expressed in Table I.
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