Abstract
Studies by Landsteiner and Van der Scheer 1 and by Landsteiner 2 have shown that 2 different species-hybrids may be distinguished from their parental species by the use of sera immunized against the respective blood cells, with subsequent agglutinin-absorptions. The experiments to be presented involve a cross of females of the domesticated Ring dove (Streptopelia risoria) with males of an Asiatic genus (Spilopelia chinensis), commonly called P'earlneck. Antisera were prepared by injecting rabbits with erythrocytes from individual representatives of each genus and of the hybrid. The agglutinations were performed by adding to 0.1 cc. of the immune serum in its varying dilutions (by halves) one drop of a 2.5% suspension of the red blood cells. For the absorptions, twice the volume of the serum diluted according to its original titre was added to a given volume of washed, packed red blood cells. The mixture was agitated gently at intervals, allowed to stand at room temperature for 2 hours and in the ice box overnight. All absorptions were repeated until complete at a dilution of 1:30. Readings were generally made after 2 hours at room temperature.
Without giving the inter-agglutinations of the parental genera and the hybrid, to be published elsewhere, it may be said that each was readily distinguishable from the others after reciprocal absorptions had been made.
Certain serological inter-relationships of the parental genera and the hybrid are presented in the table. On the basis of these reactions, it is seen that the cells of the hybrid resemble those of the Pearlneck genus more closely than those of the Ring dove.
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