Abstract
A method has already been described 1 for the separation from liver of an acidic substance clinically potent in pernicious anemia. The substance on hydrolysis was found to yield β-hydroxyglutamic acid and evidence was also obtained of the presence in the hydrolytic products of a neutral laevorotatory compound precipitable at least in part by phototungstic acid. This latter substance has been identified as l-γ-hydroxyproline. It was characterized by its specific laevorotation, absence of amino nitrogen, phenylisocyanate derivative M. P. 170° and copper salt. The free acid appears identical with the product obtained by the hydrolysis of proteins. The mode of linkage of the hydroxyproline with hydroxyglutamic acid is under investigation.
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