Abstract
If 1 cc. horse serum is added to 19 cc. normal dog serum and the mixture is heated to 60° C. for several days, parallel titrations of the resulting products by means of ice-chest ripened anti-horse rabbit precipitin show a gradual flattening of the precipitin graph, 1 without appreciable change in the end reaction to “titer”. The flattening of the graph is, therefore, taken as an index of specific protein denaturization. The stability of the end point suggests the relative reliability of the end reaction as a quantitative test.
The above tests are preliminary to an attempted interpretation of the altered precipitin graphs obtained with parenterally injected alien proteins.
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