Abstract
If 5 cc. 7.5% horse serum-globulin are added to 95 cc. normal dog serum, the mixture allowed to stand in the ice chest over night, and the proteins of the mixture then separated by half-saturation, followed by full-saturation with ammonium sulphate, titrations by means of specific rabbit precipitin indicate a quantitative recovery of the horse protein in the globulin fraction of the serum mixture.
If horse serum-albumin is similarly added to normal dog serum, 331/3% of the horse protein is recovered in the globulin fraction, and 662/3% is the albumin fraction. A similar apparent 331/3% conversion of horse albumin into horse globulin takes place in heat-inactivated normal dog serum.
The above tests are preliminary to a study of the mechanism of the intravenous denaturization of foreign proteins.
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