Abstract
Conclusion
It is possible to obtain highly potent skin preparatory and reacting factors in filtrates of washings of 20-22 hour old Bacillus typhosus cultures on plain agar. Under these conditions there is avoided much of the extraneous material present in tryptic digest broth previously employed, as well as much of the bacterial autolysis occurring in the older method. This would indicate that the skin preparatory and reacting factors are bacterial soluble substances, the production of which is independent of massive cell destruction.
The filtrates prepared in this manner produce negligible primary effect upon the skin (erythema, swelling), notwithstanding their high skin preparatory potency. This is in agreement with the view previously expressed 1 , 2 5 that the skin preparatory potency of a given filtrate bears no relation to its primary effect on the skin.
It is of considerable interest to note that there exists a distinct reciprocal quantitative relationship between the skin and intravenous (loses. This observation merits further study.
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