Abstract
The mannitol medium used is ordinary agar to which 4% mannitol has been added. Large tubes are used, not the ordinary culture tubes employed for bacterial cultures. The tubes after inoculation are kept at 26° or in New Orleans at the temperature of the room for 3 weeks. Two to 3 weeks after inoculation certain blastomycoides fungi produce black pigmentation of the medium constantly, others occasionally, others never produce any pigmentation. To the first group belongs Blastomycoides immitis, to the second Blastomycoides dermatitidis, to the third Blastomycoides tulanensis.
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