Abstract
During the past year Kelser 1 has prepared a chloroform-treated tissue-vaccine for rinderpest which is highly efficacious in protecting cattle and carabaos against this disease. Kakisaki, Nakanishi and Nakamura 2 in Japan have also prepared a tissue-vaccine for this disease by treating the infected tissues with toluol, ether, etc. The vaccine prepared by the Japanese workers is difficult to administer because of its consistency and is also absorbed very slowly. In the Philippines the Kelser vaccine has now been adopted as the standard method of protection against rinderpest and its efficiency approaches 100%. In more recent work Kelser 3 has prepared a chloroform-treated tissue-vaccine for rabies.
Briefly the chloroform-treated tissue vaccine for rinderpest as prepared by Kelser consists of finely ground tissues (lymph glands, tonsils, spleen, liver) from animals killed in the acute stages of rinderpest. This tissue is diluted with equal parts of physiological saline and for each 100 cc. of tissue emulsion 0.75 cc. of chloroform is added. Such a vaccine can be used a few hours after preparation. Kelser prepared a tissue vaccine for rabies by the same method except that in this case he employed the infected brains of rabbits dying of rabies. Vaccinated rabbits were found to be immune to subdural inoculation of rabies virus after 2 and 3 subcutaneous injections of the chloroform-treated brain emulsion.
Get full access to this article
View all access options for this article.