Abstract
Previously reported 1 , 2 , 3 experiments on the effect of nuclear extracts in anemias have led us to an extension of the source of material elsewhere than to fowl's blood and beef liver. The present report will deal with the hemopoietic effect of extracts obtained by the methods of Hammarsten and of Kossel-Neumann from kidney, pancreas and spleen on experimental rabbits, and with the effect of similarly obtained extracts from liver and spleen on pernicious anemia in human patients.
Experimental anemia was produced in rabbits by repeated injections of water intravenously. Six control animals were run without further treatment, after a degree of anemia corresponding to that of the treated animals had been produced. One of the controls died, apparently as a result of the water injection. Averages for the 5 remaining show an increase from 3,638,000 to 6,040,000 erythrocytes per cm. in 26 days. This result corresponds closely with that from a previous series 3 of controls similarly treated. The reticulocytes did not increase beyond 3%.
A second series of rabbits was treated with extract from beef kidney. Two of these animals were treated without having been made anemic. They showed an increase from 5,850,000 to 6,580,000 and from 5,670,000 to 6,876,000 erythrocytes per c. mm., respectively, in 6 days. On the 6th day the reticulocytes numbered 12% in each case. The erythrocyte count continued to increase to a high point of 7,620,000 and 7,680,000 per c. mm., respectively, in 21 days. The hemoglobin showed corresponding increases. Two anemic rabbits treated with extract from kidney showed an increase in erythrocyte count from 3,640,000 to 6,980,000 and from 3,600,-000 to 6,580,000 per c. mm., respectively, in 12 days. The hemoglobin increased from 58% to 112% in one case and from 52% to 115% in the second rabbit during the same period. The high point in reticulocyte percentage was 12% and 10%, respectively, in each case on the 5th day.
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